Journal: BMC Genomics

Article Title: Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum

doi: 10.1186/1471-2164-13-445

Figure Lengend Snippet: Genetic map of the sigH-rshA operon, its Northern hybridization analysis in C. glutamicum RES167 and its deletion derivatives. A . Genetic map of the sigH-rshA region showing locations and sizes of deletions in the chromosomes of strains C. glutamicum Δ rshA , DN2 and AS1, predicted sizes of respective sigH-rshA and rshA transcripts (arrows) and locations of probes used for Northern hybridizations. Promoters are indicated with bent arrows and the terminator with a hairpin symbol. B . Northern blot using a sigH probe (left panel) and an rshA probe (right panel) hybridized with total RNA extracted from: RES167 cells (lane 1); DN2 cells (Δ sigH deletion; lane 2); AS1 cells (Δ sigHrshA deletion; lane 3). The estimated lenghts of the detected transcripts (left) and their designations (right) are indicated. The sizes of the fragments in the RNA marker are indicated with arrows.

Article Snippet: The blotting was performed with total RNA prepared from C. glutamicum RES167 (restriction-deficient variant derived from the ATCC 13032 type strain and its derived deletion mutant strains DN2 (carrying a deletion within sigH ) and AS1 (carrying a complete deletion of sigHrshA ).

Techniques: Northern Blot, Hybridization, Marker

Journal: BMC Genomics

Article Title: Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum

doi: 10.1186/1471-2164-13-445

Figure Lengend Snippet: Microarray analysis of the C. glutamicum RES167 strain compared with its Δ rshA mutant DN2. Ratio/intensity plot obtained from the DNA microarray comparing the transcriptomes of RES167 and DN2 is shown. Total RNA was isolated from two biological replicates grown in minimal CGXII medium to the exponential phase and used for hybridization. Genes with increased amounts of mRNA in the Δ rshA strain have positive ratios, while genes with a higher mRNA amount in the RES167 strain have negative ratios, indicated with green diamonds (upregulated) or red triangles (downregulated) respectively; those not exhibiting differential expression are indicated with grey spots. M values of higher than +0.6 or lower than −0.6 (corresponding to fold changes of 1.52 and 0.66, respectively) were considered to be significant. The relevant genes are indicated by their names or desigations from the C. glutamicum genome sequence (GenBank NC_006958), underlined genes were previously described as SigH-dependent.

Article Snippet: The blotting was performed with total RNA prepared from C. glutamicum RES167 (restriction-deficient variant derived from the ATCC 13032 type strain and its derived deletion mutant strains DN2 (carrying a deletion within sigH ) and AS1 (carrying a complete deletion of sigHrshA ).

Techniques: Microarray, Mutagenesis, Isolation, Hybridization, Quantitative Proteomics, Sequencing

Journal: BMC Genomics

Article Title: Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum

doi: 10.1186/1471-2164-13-445

Figure Lengend Snippet: Genes with enhanced expression in C. glutamicum Δ rshA compared with C . glutamicum RES167 (reference) sorted by function

Article Snippet: The blotting was performed with total RNA prepared from C. glutamicum RES167 (restriction-deficient variant derived from the ATCC 13032 type strain and its derived deletion mutant strains DN2 (carrying a deletion within sigH ) and AS1 (carrying a complete deletion of sigHrshA ).

Techniques: Expressing, Sequencing, Methylation, Ubiquitin Proteomics, Membrane, Luciferase

Journal: BMC Genomics

Article Title: Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum

doi: 10.1186/1471-2164-13-445

Figure Lengend Snippet: Relative transcript levels of selected potential SigH-dependent genes in C. glutamicum Δ rshA/ C. glutamicum RES167 measured by q-RT-PCR. The data obtained for the RES167 strain served as a reference and the respective values were set to 1.0 on the logarithmic scale. Three biological replicates for the Δ rshA strain and four replicates for the RES167 strain were analysed in duplicate. SD values are shown as error bars.

Article Snippet: The blotting was performed with total RNA prepared from C. glutamicum RES167 (restriction-deficient variant derived from the ATCC 13032 type strain and its derived deletion mutant strains DN2 (carrying a deletion within sigH ) and AS1 (carrying a complete deletion of sigHrshA ).

Techniques: Reverse Transcription Polymerase Chain Reaction

Journal: BMC Genomics

Article Title: Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum

doi: 10.1186/1471-2164-13-445

Figure Lengend Snippet: Plasmids and bacteria used in this work

Article Snippet: The blotting was performed with total RNA prepared from C. glutamicum RES167 (restriction-deficient variant derived from the ATCC 13032 type strain and its derived deletion mutant strains DN2 (carrying a deletion within sigH ) and AS1 (carrying a complete deletion of sigHrshA ).

Techniques: Bacteria, Plasmid Preparation